ANTIBODY AFFINITY EXTRACTION MS

Brand	Owner	Address	Description
AAE-MS	CYGNUS TECHNOLOGIES	4332 Southport Supply Rd., SE Southport NC 28461	ANTIBODY AFFINITY EXTRACTION-MS;Scientific research and development, namely, scientific research and development service in the field of identifying and analyzing proteins;
	Where the owner name is not linked, that owner no longer owns the brand

Technical Examples

The present invention relates to a method for adjusting the affinity of a polypeptide to a target molecule by a combination of steps, including: (1) the identification of aspartyl residues which are prone to isomerization; (2) the substitution of alternative residues and screening the resulting mutants for affinity against the target molecule. In a preferred embodiment, the method of subtituting residues is affinity maturation with phage display (AMPD). In a further preferred embodiment the polypeptide is an antibody and the target molecule is an antigen. In a further preferred embodiment, the antibody is anti-IgE and the target molecule is IgE. In another embodiment, the invention relates to an anti-IgE antibody having improved affinity to IgE.