AFFINITY CHROMATOGRAPHY COLUMN

Brand	Owner	Address	Description
ECOLOGIENA	Takeda Chemical Industries, Ltd.	1-1, Doshomachi 4-Chome, Chuo-Ku Osaka Japan	affinity chromatography column;antibody bound absorbent for concentration and cleaning of sample water and fluids;filters and filtering apparatus for sample water and fluids;
	Where the owner name is not linked, that owner no longer owns the brand

Technical Examples

The present invention provides a process for dissociating Fc-containing molecules from complexes of Protein A/Fc-containing molecules or mixtures containing Fc-containing molecules and Protein A. The association, e.g., by hydrophobic interactions, between the Fc-containing molecules and Protein A can be reduced or inhibited by raising the pH of dissociation. The pH of dissociation can be raised by addition of agents capable of inhibiting hydrophobic interactions, including buffers containing arginine and/or ethylene glycol, to the mixture, either prior to adding the mixture to the column chromatography substrate, after adding the mixture to the column chromatography substrate, or both prior to and after adding the mixture to the column chromatography substrate. Separation of Fc-containing molecules from Protein A can be performed on a number of different column chromatographic substrates, including column chromatographic substrates contained in Q columns (Q column chromatography substrate), HIC columns (hydrophobic interaction column chromatography substrate), and IMAC columns (metal chelate column chromatography substrate).