CHROMOGENIC SITU HYBRIDIZATION PROBES

Brand	Owner	Address	Description
RISH	BIOCARE MEDICAL	4040 Pike Lane Concord CA 94520	Chromogenic in situ hybridization probes, namely, DNA probes for identifying medically significant genes and gene expressions; Chromogenic in situ hybridization detection kits for the diagnosis of disease consisting of enzymes and reagents for pretreatment of tissues, DNA probes, stringent and non-stringent wash buffers, anti-digoxigenin or anti-biotin antibodies, secondary detection reagents, polymers, tertiary detection reagents, and chromogens;
	Where the owner name is not linked, that owner no longer owns the brand

Technical Examples

The conditions under which oligonucleotide probes hybridize preferentially with entirely complementary and homologous nucleic acid targets are described. Using these hybridization conditions, overlapping oligonucleotide probes associate with a target nucleic acid. Following washes, positive hybridization signals are used to assemble the sequence of a given nucleic acid fragment. Representative target nucleic acids are applied as dots. Up to to 100,000 probes of the type (A,T,C,G)(A,T,C,G)NB(A,T,C,G) are used to determine sequence information by simultaneous hybridization with nucleic acid molecules bound to a filter. Additional hybridization conditions are provided that allow stringent hybridization of 6-10 nucleotide long oligomers which extends the utility of the invention. A computer process determines the information sequence of the target nucleic acid which can include targets with the complexity of mammalian genomes. Sequence generation can be obtained for a large complex mammalian genome in a single process.